Difference between revisions of "Part:BBa K4073001"

 
 
Line 11: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4073001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4073001 SequenceAndFeatures</partinfo>
 
+
https://static.igem.org/mediawiki/parts/9/9b/T--WVHS_SanDiego_CA--strongRBSnew.png
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 21:55, 21 October 2021


Promoter expresses genes when the salt threshold is reached.

This promoter is found in C. reinhardtii, a green microalga species. In C. reinhardtii, the promoter expresses the CrGDPH3 gene under NaCl treatments, so the name of the promoter is the CrGDPH3 promoter. This promoter can be used for any project that requires salt-inducible regulation of certain genes. Unfortunately, we were unable to obtain a labspace to determine the experimental results of this promoter on enhancing salinity mechanisms. With a lab, we would have collected quantitative data from experiments on the CrGDPH3 promoter, such as data regarding the required threshold of NaCl to trigger expression of transgenes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225

T--WVHS_SanDiego_CA--strongRBSnew.png