Difference between revisions of "Part:BBa K3866020"
Line 3: Line 3:
 
<partinfo>BBa_K3866020 short</partinfo>
 
<partinfo>BBa_K3866020 short</partinfo>
  
−
[[File:T--Thessaly--omega2.CymR.Stuffer.png|700px|thumb|none|<i><b>Fig.2:</b>The omega 2 vector with two translational units. The one is J23115-CymR-double terminator and the other one is ths stuffer.</i>]]
+
[[File:T--Thessaly--omega2.CymR.Stuffer.png|700px|thumb|none|<i><b>Fig.2:</b>The omega 2 vector with two translational units. The one is J23115-CymR-double terminator and the other one is the stuffer.</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:31, 26 September 2021


J23115-CymR-Stuffer a2

Fig.2:The omega 2 vector with two translational units. The one is J23115-CymR-double terminator and the other one is the stuffer.

Usage and Biology

This composite part is consisted of the translational unit of the strong constitutive promoter J23115 BBa_K3505012and the Cym Repressor BBa_K415202and the transcriptional unit with the stuffer BBa_K3866019, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector BBa_K3505011.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 418
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101

Source

Synthesized by Twist Biosciences.

References

i