Difference between revisions of "Part:BBa K3766013"
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<partinfo>BBa_K3766013 short</partinfo> | <partinfo>BBa_K3766013 short</partinfo> | ||
− | mCherry | + | This codon optimized mCherry for ''Escherichia coli'' K12 is the [[Part:BBa_K2448004|BBa_K2448004]] in reverse orientation. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | mCherry [1] is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from ''Discosoma'' sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching. | ||
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+ | ===References=== | ||
+ | [1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from ''Discosoma'' sp. red fluorescent protein. Nature Biotechnology (2004) 22: 1567–1572. | ||
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Latest revision as of 22:26, 16 October 2021
mCherry (reverse orientation)
This codon optimized mCherry for Escherichia coli K12 is the BBa_K2448004 in reverse orientation.
Usage and Biology
mCherry [1] is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching.
References
[1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology (2004) 22: 1567–1572.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 137 - 1000COMPATIBLE WITH RFC[1000]