Difference between revisions of "Part:BBa K3766012:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3766012 short</partinfo> | ||
+ | |||
+ | <partinfo>BBa_K3766012 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | |||
+ | To disrupt the BsaI recognition site GGTCTC by site-directed mutagenesis we choose to change its sequence to GGTCGC. Care was taken that the mutation should not affect the amino acid composition of the protein. The chosen mutation is a synonymous one TCT(Ser) to TCG(Ser). | ||
+ | |||
+ | >BsaI | ||
+ | | | ||
+ | pSB1A2: 1103 - GGG TCT CGC - 1095 | ||
+ | G S R | ||
+ | |||
+ | BBa K3766012: 715 - GGG TCG CGC - 723 | ||
+ | G S R | ||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | PCR amplification from a BsaI-free version of pSB1A2 | ||
+ | |||
+ | ===References=== |
Latest revision as of 23:36, 25 September 2021
AmpR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To disrupt the BsaI recognition site GGTCTC by site-directed mutagenesis we choose to change its sequence to GGTCGC. Care was taken that the mutation should not affect the amino acid composition of the protein. The chosen mutation is a synonymous one TCT(Ser) to TCG(Ser).
>BsaI | pSB1A2: 1103 - GGG TCT CGC - 1095 G S R BBa K3766012: 715 - GGG TCG CGC - 723 G S R
Source
PCR amplification from a BsaI-free version of pSB1A2