Difference between revisions of "Part:BBa K3866020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p>This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart> and the Cym Repressor <bbpart> | + | <p>This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart> and the Cym Repressor <bbpart>BBa_K415202</bbpart> and the transcriptional unit with the stuffer <bbpart>BBa_K3866019</bbpart>, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector <bbpart>BBa_K3505011</bbpart>. |
===Design Notes=== | ===Design Notes=== | ||
Revision as of 17:39, 25 September 2021
J23115-CymR-Stuffer a2
Usage and Biology
This composite part is consisted of the translational unit of the strong constitutive promoter J23115 BBa_K3505012 and the Cym Repressor BBa_K415202 and the transcriptional unit with the stuffer BBa_K3866019, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector BBa_K3505011.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 418
Illegal XhoI site found at 199 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Source
Synthesized by Twist Biosciences.