Difference between revisions of "Part:BBa K3866016"
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<partinfo>BBa_K3866016 short</partinfo> | <partinfo>BBa_K3866016 short</partinfo> | ||
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− | + | [[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]] | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | This part includes the weak promoter of the lactose operon without the lactose operator,which is used for protein expression. Also, it includes the antitoxin MazE that will be utilized to the toxin-antitoxin system and the double terminator. | |
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+ | ===Design Notes=== | ||
+ | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for GoldenBraid cloning. | ||
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+ | ===Sequence and Features=== | ||
<partinfo>BBa_K3866016 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3866016 SequenceAndFeatures</partinfo> | ||
+ | ===Source=== | ||
+ | Synthesized by Twist Biosciences. | ||
− | + | ===References=== | |
− | === | + | i |
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Revision as of 17:20, 25 September 2021
Plac-MazE-double terminator
Usage and Biology
This part includes the weak promoter of the lactose operon without the lactose operator,which is used for protein expression. Also, it includes the antitoxin MazE that will be utilized to the toxin-antitoxin system and the double terminator.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
Synthesized by Twist Biosciences.
References
i