Difference between revisions of "Part:BBa K3866002"

 
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<partinfo>BBa_K3866002 short</partinfo>
 
<partinfo>BBa_K3866002 short</partinfo>
  
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===Usage and Biology===
 
===Usage and Biology===
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Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.
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===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning.
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The CDS has position B3-B5.
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<p style="text-align: center;">
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    [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
  
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===Verification of Cloning===
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<span class='h3bb'>Sequence and Features</span>
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[[File:T--Thessaly--laci.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2</i>]]
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<partinfo>BBa_K3866002 SequenceAndFeatures</partinfo>
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===Source===
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Synthesized by Twist Biosciences.
  
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===Sequence and Features===
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===Functional Parameters===
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<partinfo>BBa_K3866002 SequenceAndFeatures</partinfo>   9/6/2021
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<partinfo>BBa_K3866002 parameters</partinfo>
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Revision as of 11:42, 25 September 2021



ackA-Acetate kinase GB compatible with B3-B5


Usage and Biology

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2

Source

Synthesized by Twist Biosciences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
9/6/2021