Difference between revisions of "Part:BBa K4040017"
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===The structure and function of CAR ζ===
 
===The structure and function of CAR ζ===
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CAR ζ is another CAR aimed to increase the phagocytosis of the macrophages. And the structure is also similar to the other three CARs, with the difference in the intracellular domain which is replaced by CD ζ subunit of the T cell receptor.
 
CAR ζ is another CAR aimed to increase the phagocytosis of the macrophages. And the structure is also similar to the other three CARs, with the difference in the intracellular domain which is replaced by CD ζ subunit of the T cell receptor.
 
===The mechanism of CAR ζ===
 
===The mechanism of CAR ζ===
 
The main function domain of the CAR ζ is the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) as mentioned before. When the ligands matches its receptor, the Src family kinases will consequently be phosphorylated and thus activating the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs). The further signal pathway is the same as mentioned in CAR-MEGF10(<a href="https://parts.igem.org/Part:BBa_K4040015">BBa_K4040015</a>).
 
The main function domain of the CAR ζ is the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) as mentioned before. When the ligands matches its receptor, the Src family kinases will consequently be phosphorylated and thus activating the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs). The further signal pathway is the same as mentioned in CAR-MEGF10(<a href="https://parts.igem.org/Part:BBa_K4040015">BBa_K4040015</a>).
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:16, 21 September 2021


Synthetic Receptor CARζ

The structure and function of CAR ζ

CAR ζ is another CAR aimed to increase the phagocytosis of the macrophages. And the structure is also similar to the other three CARs, with the difference in the intracellular domain which is replaced by CD ζ subunit of the T cell receptor. ===The mechanism of CAR ζ=== The main function domain of the CAR ζ is the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) as mentioned before. When the ligands matches its receptor, the Src family kinases will consequently be phosphorylated and thus activating the cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs). The further signal pathway is the same as mentioned in CAR-MEGF10(BBa_K4040015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 1825
    Illegal NgoMIV site found at 1891
    Illegal NgoMIV site found at 2050
    Illegal AgeI site found at 440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168
    Illegal BsaI.rc site found at 1432
    Illegal SapI.rc site found at 1989