Difference between revisions of "Part:BBa K3853000"
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Manganese Peroxidase(MnP)is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from <i>Phanerochaete chrysosporium</i>. The gene enables our efficiently Multi-enzyme complex to degrade PE
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Manganese Peroxidase(MnP)is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from <i>Phanerochaete chrysosporium</i>. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the entire fusion protein gene sequence to improve its expression in Pichia pastoris. The Introduction of this gene helps our system effectively degrade PE
  
 
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Revision as of 10:37, 19 September 2021


Manganese Peroxidase (MnP)


Manganese Peroxidase(MnP)is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from Phanerochaete chrysosporium. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the entire fusion protein gene sequence to improve its expression in Pichia pastoris. The Introduction of this gene helps our system effectively degrade PE

Biology

Usage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]