Difference between revisions of "Part:BBa K4040011"

Revision as of 13:13, 29 September 2021

tTA

tTA(tetR-VP64) contains three core domains from N-terminal to C-terminal: DNA-binding transcriptional repressor(TetR), nuclear location sequence and VP64 transcription regulating domain. And a linker was added between DBD and NLS for providing region flexibility. tetRDBD enable binding to specific DNA sequences, so that we can use tetR-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promoter elements and CMV. The usage in our project can be found in IL-6R-TCS-tTA(BBa_K4040022).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 tTA(tetR-VP64) contains three core domains from N-terminal to C-terminal: DNA-binding transcriptional repressor(TetR), nuclear location sequence and VP64 transcription regulating domain. And a linker was added between DBD and NLS for providing region flexibility. tetRDBD enable binding to specific DNA sequences, so that we can use tetR-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promoter elements and CMV.
-The usage in our project can be found in
+The usage in our project can be found in <b>IL-6R-TCS-tTA</b>(<partinfo>BBa_K4040022</partinfo>).
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