Difference between revisions of "Part:BBa K4040011"

 
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<partinfo>BBa_K4040011 short</partinfo>
 
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tTA(tetR-VP64) contains three core domains from N-terminal to C-terminal: DNA-binding transcriptional repressor(TetR), nuclear location sequence and VP64 transcription regulating domain. And a linker was added between DBD and NLS for providing region flexibility. tetRDBD enable binding to specific DNA sequences, so that we can use tetR-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promoter elements and CMV.
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The usage in our project can be found in
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4040011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4040011 SequenceAndFeatures</partinfo>
  

Revision as of 01:41, 15 September 2021


tTA

tTA(tetR-VP64) contains three core domains from N-terminal to C-terminal: DNA-binding transcriptional repressor(TetR), nuclear location sequence and VP64 transcription regulating domain. And a linker was added between DBD and NLS for providing region flexibility. tetRDBD enable binding to specific DNA sequences, so that we can use tetR-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promoter elements and CMV. The usage in our project can be found in

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]