Difference between revisions of "Part:BBa K4035000:Design"
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | The CUP1 (BBa_M45090) sequence is the genomic sequence of the copper | + | The CUP1 (BBa_M45090) sequence is the genomic sequence of the yeast copper metallothionein 1 protein and was inserted in the pCTcon2-V5 plasmid (1) that was already containing Aga2, V5 tag and the Gal1 promoter. |
===References=== | ===References=== | ||
(1) Chao, G.; Lau, W. L.; Hackel, B. J.; Sazinsky, S. L.; Lippow, S. M.; Wittrup, K. D. Isolating and Engineering Human Antibodies Using Yeast Surface Display. Nat Protoc 2006, 1 (2), 755–768. https://doi.org/10.1038/nprot.2006.94. | (1) Chao, G.; Lau, W. L.; Hackel, B. J.; Sazinsky, S. L.; Lippow, S. M.; Wittrup, K. D. Isolating and Engineering Human Antibodies Using Yeast Surface Display. Nat Protoc 2006, 1 (2), 755–768. https://doi.org/10.1038/nprot.2006.94. | ||
| + | |||
| + | (2) https://www.uniprot.org/uniprot/P0CX80 | ||
Revision as of 20:40, 21 October 2021
Expression of CUP1 on the extracellular membrane of S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The start and stop codon of the CUP1 genomic sequence were removed in order to fuse the protein to Aga2 and V5.
Source
The CUP1 (BBa_M45090) sequence is the genomic sequence of the yeast copper metallothionein 1 protein and was inserted in the pCTcon2-V5 plasmid (1) that was already containing Aga2, V5 tag and the Gal1 promoter.
References
(1) Chao, G.; Lau, W. L.; Hackel, B. J.; Sazinsky, S. L.; Lippow, S. M.; Wittrup, K. D. Isolating and Engineering Human Antibodies Using Yeast Surface Display. Nat Protoc 2006, 1 (2), 755–768. https://doi.org/10.1038/nprot.2006.94.