Difference between revisions of "Part:BBa K3739052"

 
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<partinfo>BBa_K3739052 short</partinfo>
 
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Coding the fusion protein of plastic adhesive protein LC1KR-2 and green fluorescent protein.
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We anchor LCI-KR2 protein onto membranes through OmpA to stick the engineered bacteria on the polypropylene. We use <partinfo>BBa_K3739052</partinfo> to construct the expression system and anchor LCI-KR2 on the surface of VnDX.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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===Biology===
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OmpA is an anchor protein from E. coli, also find in Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCI-KR2 is fused at C terminal with Lpp-OmpA so that LCI-KR2 can be displayed on the surface of VnDX.
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===Characterization===
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====1. Identification====
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:38, 20 October 2021


J23100-B0030-OmpA-LC1KR-2-GFP-B0010

We anchor LCI-KR2 protein onto membranes through OmpA to stick the engineered bacteria on the polypropylene. We use BBa_K3739052 to construct the expression system and anchor LCI-KR2 on the surface of VnDX.

Biology

OmpA is an anchor protein from E. coli, also find in Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCI-KR2 is fused at C terminal with Lpp-OmpA so that LCI-KR2 can be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 494
    Illegal SapI.rc site found at 1109