Difference between revisions of "Part:BBa K4035004"

 
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<partinfo>BBa_K4035004 short</partinfo>
 
<partinfo>BBa_K4035004 short</partinfo>
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. Normally expressed intracellularly, CUP1 was fused to the A-agglutinin-binding subunit Aga2 (BBa_K416000) in order to be displayed on the outter yeast cell surface. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a rigid linker and is made of seven successive AP amino acid sequences. This fusion protein also contained a V5 tag in order to check its expression by Western Blot and Immunostaining. The expression was under the control of the Gal1 promoter, so that the protein was expressed only in the presence of galactose.  
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This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of seven times the AP amino acid sequence.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035011). The (AP)7 linker is a common rigid linker made of several successive Alanin followed by Proline.
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<span class='h3bb'>Sequence and Features</span>
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===Characterization===
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===Sequence and Features===
 
<partinfo>BBa_K4035004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4035004 SequenceAndFeatures</partinfo>
  

Revision as of 05:58, 13 September 2021


Dimerization of the copper metallothionein 1 : CUP1-(AP)7-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a rigid linker made of seven times the AP amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035011). The (AP)7 linker is a common rigid linker made of several successive Alanin followed by Proline.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 90
    Illegal PstI site found at 312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 90
    Illegal PstI site found at 312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 90
    Illegal PstI site found at 312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 90
    Illegal PstI site found at 312
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 306