Difference between revisions of "Part:BBa K4035003"

 
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<partinfo>BBa_K4035003 short</partinfo>
 
<partinfo>BBa_K4035003 short</partinfo>
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. Normally expressed intracellularly, CUP1 was fused to the A-agglutinin-binding subunit Aga2 (BBa_K416000) in order to be displayed on the outter yeast cell surface. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a flexible linker and is made of four successive GGGGS amino acid sequence. This fusion protein also contained a V5 tag in order to check its expression by Western Blot and Immunostaining. The expression was under the control of the Gal1 promoter, so that the protein was expressed only in the presence of galactose.  
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This protein is made of two yeast copper metallotionein protein, CUP1 ((BBa_M45090), linked together by a flexible linker made of four times the GGGGS amino acid sequence.  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035010). The GGGGS linker is a common flexible linker.
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<span class='h3bb'>Sequence and Features</span>
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===Characterization===
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===Sequence and Features===
 
<partinfo>BBa_K4035003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4035003 SequenceAndFeatures</partinfo>
  

Revision as of 05:39, 13 September 2021


Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)4-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 ((BBa_M45090), linked together by a flexible linker made of four times the GGGGS amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035010). The GGGGS linker is a common flexible linker.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 90
    Illegal PstI site found at 408
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 90
    Illegal PstI site found at 408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 90
    Illegal PstI site found at 408
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 90
    Illegal PstI site found at 408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84