Difference between revisions of "Part:BBa K3739025"

 
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<partinfo>BBa_K3739025 short</partinfo>
 
<partinfo>BBa_K3739025 short</partinfo>
  
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Anchor LC1KR-2 onto membranes through OmpA and use GFP to comformation.
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We anchor LCI-KR2 protein onto membranes through OmpA to stick the engineered bacteria on the polypropylene. We use <partinfo>BBa_K3739052</partinfo> to construct the expression system and anchor LCI-KR2 on the surface of VnDX.
  
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===Biology===
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OmpA is an anchor protein from E. coli, also find in Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCI-KR2 is fused at C terminal with Lpp-OmpA so that LCI-KR2 can be displayed on the surface of VnDX.
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===Usage and Biology===
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===Characterization===
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====1. Identification====
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
  
 
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Revision as of 18:37, 20 October 2021


OmpA-LC1KR2-GFP

We anchor LCI-KR2 protein onto membranes through OmpA to stick the engineered bacteria on the polypropylene. We use BBa_K3739052 to construct the expression system and anchor LCI-KR2 on the surface of VnDX.

Biology

OmpA is an anchor protein from E. coli, also find in Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCI-KR2 is fused at C terminal with Lpp-OmpA so that LCI-KR2 can be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430
    Illegal SapI.rc site found at 1045