Difference between revisions of "Part:BBa K3731000"
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<b>Group: Nanjing-China 2021</b>
 
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<b>Author: Haoru Song</b>
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<b>Author: Hao Yin</b>
 
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We
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Polyphosphate kinase(PPK) polymerizes the terminal phosphate of ATP to a long chain of polyphosphate(polyP) in a freely reversible reaction. Here polyphosphate kinase was purified and characterized by SDS-PAGE. Bands of 69kDa showed the existence of PPK in the Escherichia coli.
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<h2>iGEM2021_Nanjing China Experiment</h2>
 
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<p>This year our team develops a simple solo medium-copy plasmid-based polyphosphate kinase (PPK1) overexpression strategy for achieving maximum intracellular polyphosphate accumulation.</p>
 
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<p>We test</p>
 
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Revision as of 15:15, 13 September 2021


ppk1 in E.Coli BL21

In bacteria, polyP is synthesized by polyphosphate kinase (PPK). PPK consists of PPK1 and PPK2. PPK1 can lengthen the polymer by using the γ-Pi phosphate bond of ATP, and its reversible reaction is to synthesize ATP from ADP and Pi. PPK2 can use polyP to synthesize GTP or ATP reversibly, and mainly synthesize GTP. The ppk1 codes PPK1, which can promote the synthesis(major function) and decomposition(minor function) of polyP with the residue of ATP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2021_Nanjing-China Experiment

Group: Nanjing-China 2021
Author: Hao Yin

Polyphosphate kinase(PPK) polymerizes the terminal phosphate of ATP to a long chain of polyphosphate(polyP) in a freely reversible reaction. Here polyphosphate kinase was purified and characterized by SDS-PAGE. Bands of 69kDa showed the existence of PPK in the Escherichia coli.

PolyP


Reference: