Difference between revisions of "Part:BBa K138000"
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The NifH promoer is from K.pneumoniae; for some reason, I can't change the title of this part, so that annoying "check" thing is there. | The NifH promoer is from K.pneumoniae; for some reason, I can't change the title of this part, so that annoying "check" thing is there. | ||
The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI. | The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI. | ||
Revision as of 21:18, 4 November 2008
NifHp in BioBrick Compatible Leucine Landing Pad
The NifH promoer is from K.pneumoniae; for some reason, I can't change the title of this part, so that annoying "check" thing is there.
The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]