Difference between revisions of "Part:BBa K3905000"
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Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state. | Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state. | ||
| + | [[File:T--City of London UK--Complex P2.png|200px|thumb|left|diagram of switch in action.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:07, 21 October 2021
gen1 517-5p Toehold Switch
Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
File:T--City of London UK--Complex P2.png
diagram of switch in action.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 15
Illegal XbaI site found at 53 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 15
Illegal XbaI site found at 53 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 15
Illegal XbaI site found at 53 - 1000COMPATIBLE WITH RFC[1000]