Difference between revisions of "Part:BBa K4040000"
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4040000 short</partinfo>
 
<partinfo>BBa_K4040000 short</partinfo>
−
 
+
<h3><b>Background and structure of CAR-MEGF10</b></h3>
 
The function of CAR-MEGF10 is the same as the CAR&#947;, which increases the phagocytosis of the macrophages. And the structure is also highly similar to the CAR&#947;, with the only difference lying in the intracellular domain of the CAR, in which the common &#947; subunit of Fc receptors is replaced by MEGF10.
 
The function of CAR-MEGF10 is the same as the CAR&#947;, which increases the phagocytosis of the macrophages. And the structure is also highly similar to the CAR&#947;, with the only difference lying in the intracellular domain of the CAR, in which the common &#947; subunit of Fc receptors is replaced by MEGF10.
−
[[File:SMMU-China-CAR-MERTK2.png|450px|thumb|center|Figure 4. SDS-PAGE gel showing periplasmic purification of 6GIX with six different signal peptides, no signal peptide, and an empty vector control. The marker used is the NEB colour protein standard. The arrow denotes correct band size of 21 kDa for the 6GIX protein.]]
+
<h3><b>The mechanism of CAR-MEGF10</b></h3>
−
<h3><b>THIS IS A SUBTITLE</b></h3>
+
After the extracellular αS-scFv domain binds to the S protein of SARS-CoV-2, the intracellular cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) are activated with the help of the Src family kinases. And the SH2 binding domains for the protein expressing SH2 matching domains will be further exposed due to the space conformation change. The protein expressing SH2 matching domains, protein Syk, a phagocytic signaling effector can then active the downstream signaling transduction cascades and initiate the phagocytosis in our CARγ-macrophages eventually.
  
−
==THIS IS A TITLE==
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
===Usage and Biology===
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4040000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4040000 SequenceAndFeatures</partinfo>
  
−
===References===
 
−
Freudl, R. (2018, March 29). Signal peptides for recombinant protein secretion in bacterial expression systems. Microbial Cell Factories. BioMed Central Ltd. https://doi.org/10.1186/s12934-018-0901-3
 
−
 
−
Sørensen, H. P., & Mortensen, K. K. (2005). Advanced genetic strategies for recombinant protein expression in Escherichia coli. Journal of Biotechnology, 115(2), 113–128. https://doi.org/10.1016/j.jbiotec.2004.08.004
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
−
<partinfo>BBa_K4040000 parameters</partinfo>
 
 
<!-- -->
 
<!-- -->

Revision as of 03:34, 3 September 2021


Intracellular Domain of the MEGF10 Protein

Background and structure of CAR-MEGF10

The function of CAR-MEGF10 is the same as the CARγ, which increases the phagocytosis of the macrophages. And the structure is also highly similar to the CARγ, with the only difference lying in the intracellular domain of the CAR, in which the common γ subunit of Fc receptors is replaced by MEGF10.

The mechanism of CAR-MEGF10

After the extracellular αS-scFv domain binds to the S protein of SARS-CoV-2, the intracellular cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) are activated with the help of the Src family kinases. And the SH2 binding domains for the protein expressing SH2 matching domains will be further exposed due to the space conformation change. The protein expressing SH2 matching domains, protein Syk, a phagocytic signaling effector can then active the downstream signaling transduction cascades and initiate the phagocytosis in our CARγ-macrophages eventually.