Difference between revisions of "Part:BBa K079020:Experience"
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K079020 is a closed-loop LacI Reporter where GFP expression is auto-regulated. | K079020 is a closed-loop LacI Reporter where GFP expression is auto-regulated. | ||
It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit). | It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit). | ||
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[[Image:ClosedLooop.jpg |500 px|center |thumbnail| K079020 Fluorescence Image from O/N culture]] | [[Image:ClosedLooop.jpg |500 px|center |thumbnail| K079020 Fluorescence Image from O/N culture]] | ||
Revision as of 14:29, 4 November 2008
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Applications of BBa_K079020
K079020 is a closed-loop LacI Reporter where GFP expression is auto-regulated.
It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit).
The analysis result by Visual Fluo Bacteria is reported in the following table:
User Reviews
UNIQdd57da984f64c44f-partinfo-00000000-QINU UNIQdd57da984f64c44f-partinfo-00000001-QINU