Difference between revisions of "Part:BBa K3971011"

Revision as of 08:25, 12 August 2021

cscB (sucrose permease) for sucrose transport.

Usage and Biology

CscB (sucrose permease) is a sucrose/H+ symporter from E.coli that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity.

The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1].

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 481
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 525

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3971011 short</partinfo>
 ===Usage and Biology===
+CscB (sucrose permease) is a sucrose/H+ symporter from E.coli that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity.
+The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1].
 ===References===