Difference between revisions of "Part:BBa K3971007"

Revision as of 18:14, 20 October 2021

Portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome.

This part is a portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 353
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 353
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 353
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 353
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 143

Revision as of 17:40, 10 August 2021 (view source) Misaalbedi (Talk \| contribs) ← Older edit		Revision as of 18:14, 20 October 2021 (view source) Misaalbedi (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3971007 short</partinfo>		<partinfo>BBa_K3971007 short</partinfo>

−	This part is a portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid.	+	This part is a portion of the Neutral Site 1 of the <i>Synechococcus elongatus</i> UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters.