Difference between revisions of "Part:BBa K4033002"
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ZhuJ is a CYC2 that promotes the regiospecific C5-C14 second-ring cyclization for non-reduced polyketide chains of the R1128 family.[1] | ZhuJ is a CYC2 that promotes the regiospecific C5-C14 second-ring cyclization for non-reduced polyketide chains of the R1128 family.[1] | ||
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<b><font size="3">Biology and Usage</font></b> | <b><font size="3">Biology and Usage</font></b> | ||
Revision as of 10:35, 10 August 2021
ZhuJ
ZhuJ is a CYC2 that promotes the regiospecific C5-C14 second-ring cyclization for non-reduced polyketide chains of the R1128 family.[1]
Biology and Usage
ZhuJ and OKS encodes the aromatase that catalyzes the C5-C14 second-ring closure, which spontaneously leads C2-C15 to form the third-ring, and produce a 2.7-fold increase in flavokermesic acid levels(Fig.a). ZhuJ is proposed to catalyze the condensation between C5 and C14 to yield intermediate SEK4, which is the byproduct of the reaction. After the cyclization of the third-ring (C2-C15) forms spontaneously, flavokermesic acid anthrone forms.[2]
ZhuI and ZhuJ are usually used together for typeⅡPKS reconstitution.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 312
Illegal NgoMIV site found at 594 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 160
Experimental approach
References
[1] Ames, B. D., Lee, M. Y., Moody, C., Zhang, W., Tang, Y., & Tsai, S. C. (2011). Structural and biochemical characterization of ZhuI aromatase/cyclase from the R1128 polyketide pathway. Biochemistry, 50(39), 8392–8406. https://doi.org/10.1021/bi200593m
[2] Frandsen, R.J.N., Khorsand-Jamal, P., Kongstad, K.T. et al. Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans. Sci Rep 8, 12853 (2018). https://doi.org/10.1038/s41598-018-30816-9