Difference between revisions of "Part:BBa K3971002"

Revision as of 16:49, 9 August 2021

cscA - Sucrose-6-phosphate hydrolase (Invertase) from E.coli W.

Usage and Biology

cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1].

csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.

References

1: Sabri, Suriana, Lars K. Nielsen, and Claudia E. Vickers. "Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain." Applied and environmental microbiology 79.2 (2013): 478-487.

2: Lee, Jeong Wook, et al. "Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for L-threonine production." Applied microbiology and biotechnology 88.4 (2010): 905-913.

Sequence and Features