Difference between revisions of "Part:BBa K116601"
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===Sequence and Features=== | ===Sequence and Features=== | ||
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Revision as of 13:54, 30 July 2021
HtlB (ftsH) coding region from E. coli
The HtlB (ftsH) coding region from E. coli. It can be used to degrade many different proteins.
Information contributed by City of London UK (2021)
Part information is collated here to help future users of the BioBrick registry.
Metadata:
- Group: City of London UK 2021
- Author: Julian Chen
- Summary: Added information collated from existing scientific studies
Plays a role in the quality control of integral membrane proteins. Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent sequence requirements.
Degrades C-terminal-tagged cytoplasmic proteins which are tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10SA (SsrA) stable RNA.
[1]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 219
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 586
Illegal AgeI site found at 1459 - 1000COMPATIBLE WITH RFC[1000]
References
- ↑ 'Uniprot. https://www.uniprot.org/uniprot/P0AAI3