Difference between revisions of "Part:BBa K108021"
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fis is an activator of ribosomal RNA transcription as well as other genes, as a key part in upstream up-regulation of rRNA promoters [https://pubmed.ncbi.nlm.nih.gov/2209559]
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fis is an activator of ribosomal RNA transcription as well as other genes, as a key part in upstream up-regulation of rRNA promoters
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<ref>Ross, W., J. F. Thompson, J. T. Newlands, and R. L. Gourse. 1990. “E.coli Fis Protein Activates Ribosomal RNA Transcription in Vitro and in Vivo.” The EMBO Journal 9 (11): 3733–42. https://pubmed.ncbi.nlm.nih.gov/2209559.
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</ref>.
  
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It binds to a recombinatorial enhancer helix-turn-helix sequence motif, facilitating hin-mediated DNA inversion [https://pubmed.ncbi.nlm.nih.gov/9362499] as well as binding to hundreds of transcriptionally active and inactive AT-rich sites [https://pubmed.ncbi.nlm.nih.gov/18340041] approximately half of which are located in non-coding DNA [https://pubmed.ncbi.nlm.nih.gov/16963779]
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It binds to a recombinatorial enhancer helix-turn-helix sequence motif, facilitating hin-mediated DNA inversion
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<ref>Safo, M. K., W. Z. Yang, L. Corselli, S. E. Cramton, H. S. Yuan, and R. C. Johnson. 1997. “The Transactivation Region of the Fis Protein That Controls Site-Specific DNA Inversion Contains Extended Mobile Beta-Hairpin Arms.” The EMBO Journal 16 (22): 6860–73. https://doi.org/10.1093/emboj/16.22.6860.</ref>
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as well as binding to hundreds of transcriptionally active and inactive AT-rich sites  
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<ref>Cho, B.-K., E. M. Knight, C. L. Barrett, and B. O. Palsson. 2008. “Genome-Wide Analysis of Fis Binding in Escherichia Coli Indicates a Causative Role for A-/AT-Tracts.” Genome Research 18 (6): 900–910. https://doi.org/10.1101/gr.070276.107.
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</ref>
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approximately half of which are located in non-coding DNA  
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<ref>Grainger, David C., Douglas Hurd, Martin D. Goldberg, and Stephen J. W. Busby. 2006. “Association of Nucleoid Proteins with Coding and Non-Coding Segments of the Escherichia Coli Genome.” Nucleic Acids Research 34 (16): 4642–52. https://doi.org/10.1093/nar/gkl542.
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</ref>.
  
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It prevents the initiation of oriC-mediated DNA replication [https://pubmed.ncbi.nlm.nih.gov/8836178]
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It prevents the initiation of oriC-mediated DNA replication
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<ref>Wold, S. 1996. “The Escherichia Coli Fis Protein Prevents Initiation of DNA Replication from OriC in Vitro.” Nucleic Acids Research 24 (18): 3527–32. https://doi.org/10.1093/nar/24.18.3527.
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</ref>.
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K108021 parameters</partinfo>
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Latest revision as of 17:42, 26 July 2021

Fis

global DNA-binding transcriptional dual regulator of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

fis is an activator of ribosomal RNA transcription as well as other genes, as a key part in upstream up-regulation of rRNA promoters [1].

It binds to a recombinatorial enhancer helix-turn-helix sequence motif, facilitating hin-mediated DNA inversion [2] as well as binding to hundreds of transcriptionally active and inactive AT-rich sites [3] approximately half of which are located in non-coding DNA [4].

It prevents the initiation of oriC-mediated DNA replication [5].

References

  1. ↑ Ross, W., J. F. Thompson, J. T. Newlands, and R. L. Gourse. 1990. “E.coli Fis Protein Activates Ribosomal RNA Transcription in Vitro and in Vivo.” The EMBO Journal 9 (11): 3733–42. https://pubmed.ncbi.nlm.nih.gov/2209559.
  2. ↑ Safo, M. K., W. Z. Yang, L. Corselli, S. E. Cramton, H. S. Yuan, and R. C. Johnson. 1997. “The Transactivation Region of the Fis Protein That Controls Site-Specific DNA Inversion Contains Extended Mobile Beta-Hairpin Arms.” The EMBO Journal 16 (22): 6860–73. https://doi.org/10.1093/emboj/16.22.6860.
  3. ↑ Cho, B.-K., E. M. Knight, C. L. Barrett, and B. O. Palsson. 2008. “Genome-Wide Analysis of Fis Binding in Escherichia Coli Indicates a Causative Role for A-/AT-Tracts.” Genome Research 18 (6): 900–910. https://doi.org/10.1101/gr.070276.107.
  4. ↑ Grainger, David C., Douglas Hurd, Martin D. Goldberg, and Stephen J. W. Busby. 2006. “Association of Nucleoid Proteins with Coding and Non-Coding Segments of the Escherichia Coli Genome.” Nucleic Acids Research 34 (16): 4642–52. https://doi.org/10.1093/nar/gkl542.
  5. ↑ Wold, S. 1996. “The Escherichia Coli Fis Protein Prevents Initiation of DNA Replication from OriC in Vitro.” Nucleic Acids Research 24 (18): 3527–32. https://doi.org/10.1093/nar/24.18.3527.