Difference between revisions of "Part:BBa K3767001"
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Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space (1) [[Part:BBa_K3767001#References|<sup>[1]</sup>]] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye. | Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space (1) [[Part:BBa_K3767001#References|<sup>[1]</sup>]] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[[Part:BBa_K1216001#References|<sup>[1]</sup>]]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[[Part:BBa_K1216001#References|<sup>[2]</sup>]]. | Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[[Part:BBa_K1216001#References|<sup>[1]</sup>]]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[[Part:BBa_K1216001#References|<sup>[2]</sup>]]. | ||
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<partinfo>BBa_K3767001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3767001 SequenceAndFeatures</partinfo> | ||
| + | ==Characterization== | ||
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Revision as of 14:29, 11 June 2021
Alkaline Phosphatase optimized for E. Coli w/ 40x catalytic activity
Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space (1) [1] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye.
Usage and Biology
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[1]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]