Difference between revisions of "Part:BBa K3657037"
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This part is an RNA containing binding sites for Pumby protein binding AUAGAU (<a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>) and Pumby protein binding AUGGUU (<a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>).
 
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (<a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>) and Pumby protein binding AUGGUU (<a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>).
 
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Revision as of 03:40, 28 October 2020


RNA Linker binding Pumby AUAGAU and Pumby AUGGUU
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (BBa_K3657004) and Pumby protein binding AUGGUU (BBa_K3657005).

Usage and Biology

Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. Therefore the protein can be designed to bind a specific RNA sequence.

It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: BBa_K3657034, K3657035.

Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: iGEM Heidelberg 2020

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]