Difference between revisions of "Part:BBa K3657012"
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Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657009">BBa_K3657009</a><p>
 
Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657009">BBa_K3657009</a><p>
  
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It is an ON target PPR Protein for  binding the RNA sequence ACACAG which cannot be found in the broccoli aptamers.
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It is an ON target PPR Protein for plasmides, containing the RBS <a href="https://parts.igem.org/Part:BBa_B0032">BBa_B0032</a> because it binds to the RNA sequence ACACAG, which is part of the RBS.
 +
It is an OFF target PPR Protein for the broccoli aptamer because it binds to the RNA sequence ACACAG,  which cannot be found in the broccoli aptamers.
 +
<br>
 +
For more information, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>
 
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==Characterisation==
 
==Characterisation==
  
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In combination with part BBa K3657012, an assay was conducted, messuaring
+
In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 03:12, 28 October 2020


RNA binding Pentatricopeptide Repeat Protein - Motif ACACAG


This Part is an example of a PPR Protein, assembled with the PPR Domains that are specific for the four RNA bases.

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

It is an ON target PPR Protein for plasmides, containing the RBS BBa_B0032 because it binds to the RNA sequence ACACAG, which is part of the RBS. It is an OFF target PPR Protein for the broccoli aptamer because it binds to the RNA sequence ACACAG, which cannot be found in the broccoli aptamers.
For more information, visit iGEM Heidelberg 2020

Usage and Biology

PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation

In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring