Difference between revisions of "Part:BBa K3606082"
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<partinfo>BBa_K3606082 short</partinfo> | <partinfo>BBa_K3606082 short</partinfo> | ||
| − | <p>PlacIq_lacI_Ptac driven | + | <p>PlacIq_lacI_Ptac driven DsbA-CaAP is a part of our signal-peptide guided CaAP secretion system. |
<p>To test the secretion efficiency of our 5 secretion peptides fused CaAP(Calcium Absorption Peptide) guided by 5 secretion peptides, we put them under the regulation of the iPTG inducible promoter, Ptac, and expressed them in E.coli. BL21 strain respectively. Thus, quantified characterization of these secretion peptides fused with CaAP is expected to be achieved through the different quantity of the target protein, namely CaAP, in the bacterial lysate and the culture medium.</p> | <p>To test the secretion efficiency of our 5 secretion peptides fused CaAP(Calcium Absorption Peptide) guided by 5 secretion peptides, we put them under the regulation of the iPTG inducible promoter, Ptac, and expressed them in E.coli. BL21 strain respectively. Thus, quantified characterization of these secretion peptides fused with CaAP is expected to be achieved through the different quantity of the target protein, namely CaAP, in the bacterial lysate and the culture medium.</p> | ||
<p>The 5 signal peptides candidates for CaAP secretion are listed below.</p> | <p>The 5 signal peptides candidates for CaAP secretion are listed below.</p> | ||
Latest revision as of 02:27, 28 October 2020
PlacIq_lacI_Ptac driven DsbA-CaAP
PlacIq_lacI_Ptac driven DsbA-CaAP is a part of our signal-peptide guided CaAP secretion system. <p>To test the secretion efficiency of our 5 secretion peptides fused CaAP(Calcium Absorption Peptide) guided by 5 secretion peptides, we put them under the regulation of the iPTG inducible promoter, Ptac, and expressed them in E.coli. BL21 strain respectively. Thus, quantified characterization of these secretion peptides fused with CaAP is expected to be achieved through the different quantity of the target protein, namely CaAP, in the bacterial lysate and the culture medium.
The 5 signal peptides candidates for CaAP secretion are listed below.
| Signal Peptide | Number for Registry |
|---|---|
| NSP4 | BBa_K3606042 |
| OmpA | BBa_K3606043 |
| DsbA | BBa_K3606030 |
| PelB | BBa_K208004 |
| PhoA | BBa_K808028 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1233
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]