Difference between revisions of "Part:BBa K084012:Experience"
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One part of the senders cultures were mixed with 1 part of the receiver culture. | One part of the senders cultures were mixed with 1 part of the receiver culture. | ||
| − | (Mixed sender culture | + | (Mixed sender culture 500μL with receiver culture 500μL.) |
[[image:senders_crosstalk_chiba_01.gif|frame|left| | [[image:senders_crosstalk_chiba_01.gif|frame|left| | ||
Revision as of 09:14, 30 October 2008
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K084012
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The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].
One part of the senders cultures were mixed with 1 part of the receiver culture. (Mixed sender culture 500μL with receiver culture 500μL.)
senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K0840012,BBa_K084008,BBa_K084007 respectively.
senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K0840012,BBa_K084008,BBa_K084007 respectively.
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iGEMChiba 2008 |
The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol]. |
UNIQfbdfdb1c21e2aac5-partinfo-00000001-QINU UNIQfbdfdb1c21e2aac5-partinfo-00000002-QINU