Difference between revisions of "Part:BBa K3600015:Experience"
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===Applications of BBa_K3600015=== | ===Applications of BBa_K3600015=== | ||
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| + | ===Expression of mScarlet-I in presence of atrazine=== | ||
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| + | <html> | ||
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| + | <img src="https://2020.igem.org/wiki/images/9/98/T--EPFL--ReporterStrains-Results-microscopy-YCF1.png" width="800px"/> | ||
| + | <p> | ||
| + | 1mg/L atrazine in methanol was added to overnight cultures to test if the expression of mScarletI could be induced. The final concentration of methanol is 1%. The signal was quantified using imageJ. The fluorescence signals were corrected for the background. The mean signal (n=15) for wildtype cells in the same setup was found to be at 12.1928 after correcting for the background. | ||
| + | </p> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:46, 28 October 2020
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Applications of BBa_K3600015
Expression of mScarlet-I in presence of atrazine
1mg/L atrazine in methanol was added to overnight cultures to test if the expression of mScarletI could be induced. The final concentration of methanol is 1%. The signal was quantified using imageJ. The fluorescence signals were corrected for the background. The mean signal (n=15) for wildtype cells in the same setup was found to be at 12.1928 after correcting for the background.
User Reviews
UNIQ9f2fa5614040575a-partinfo-00000001-QINU UNIQ9f2fa5614040575a-partinfo-00000002-QINU