Difference between revisions of "Part:BBa K3606807"
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McbB works as synthase responsible for the maturation of MccB17. | McbB works as synthase responsible for the maturation of MccB17. | ||
<h2>Design:</h2> | <h2>Design:</h2> | ||
| − | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the | + | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced. |
<h2>Results:</h2> | <h2>Results:</h2> | ||
Latest revision as of 02:42, 5 December 2020
mcbB
Usage and Biology:
McbB works as synthase responsible for the maturation of MccB17.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.
Results:
More information：https://parts.igem.org/Part:BBa_K3606814
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]