Difference between revisions of "Part:BBa K3408000:Design"

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===References===
 
===References===
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Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli [J]. Applied and Environmental Biology,2014,20(02):295-299.
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[1] Hwang Hee Jin,Lee Sang Yup,Lee Pyung Cheon. Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli [J]. Biotechnology for biofuels,2018,11.

Latest revision as of 01:15, 28 October 2020


promoter nar(fnr regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.

Source

The sequence is found through the paper 'Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli'.

References

[1] Hwang Hee Jin,Lee Sang Yup,Lee Pyung Cheon. Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli [J]. Biotechnology for biofuels,2018,11.