Difference between revisions of "Part:BBa K3408000:Design"
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| − | Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299. | + | Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli [J]. Applied and Environmental Biology,2014,20(02):295-299. |
Revision as of 01:09, 28 October 2020
promoter nar(fnr regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.
Source
The sequence is found through the paper 'Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli'.
References
Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli [J]. Applied and Environmental Biology,2014,20(02):295-299.