Difference between revisions of "Part:BBa K3657000"
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
 
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Revision as of 00:50, 28 October 2020


RNA binding Pumilio Homology Domain (Adenine)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.


This part is the Adenine binding Pumby Domain.


It can be assembled to a Pumby Protein when combined with the other Pumby Domains:

Guanine:BBa_K3657001

Uracil:BBa_K3657002

Cytosine:BBa_K3657003


See also the compositive parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]