Difference between revisions of "Part:BBa K3657002"
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
 
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
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This part is the Uracil binding Pumby Domain.   
 
This part is the Uracil binding Pumby Domain.   
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Combined with the other Pumby Domains (<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a>, <a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a>, <a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a>) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)
 
Combined with the other Pumby Domains (<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a>, <a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a>, <a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a>) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:08, 28 October 2020


RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.


This part is the Uracil binding Pumby Domain.


Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]