Difference between revisions of "Part:BBa K3331005"
Line 27: Line 27:
 
groups were the control group without arabinose induction, and three of 6-4 groups were  
 
groups were the control group without arabinose induction, and three of 6-4 groups were  
 
the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the  
 
the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the  
−
group with * is 10^4, and the dilution factor of the group without * is 10^6.
+
group with * is 10^4, and the dilution factor of the group without * is 10^6.
 +
 
 +
Result: The bacteria without arabinose induction grow normally, while with arabinose
 +
induction, the bacteria suicide.
 
<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b> Experimental medium. 6-1 is blank control ,6-4 is experimental group.]]</div>
 
<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b> Experimental medium. 6-1 is blank control ,6-4 is experimental group.]]</div>

Revision as of 00:06, 28 October 2020


nodA(MazF)

ndoA (ydcD, mazF) [ Bacillus subtilis subsp. subtilis str. 168 ]


Encodes EndoA, an endoribonuclease that inactivates cellular mRNAs by cleaving them at specific, but frequently occurring sites. EndoA has similar cleavage specificity to MazF and PemK and yields cleavage products with 3′‐phosphate and 5′‐hydroxyl groups, typical of EDTA‐resistant degradative RNases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

In the figure, the bacteria in this plate contained plasmids of pBAD-MazF. Three of 6-1 groups were the control group without arabinose induction, and three of 6-4 groups were the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the group with * is 10^4, and the dilution factor of the group without * is 10^6.

Result: The bacteria without arabinose induction grow normally, while with arabinose induction, the bacteria suicide.

Figure 1: Experimental medium. 6-1 is blank control ,6-4 is experimental group.