Difference between revisions of "Part:BBa K3475003"
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text-align:justify "><em><strong>FIGURE 2.</strong> '''The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).'''<br/>Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank. </em> </p> | text-align:justify "><em><strong>FIGURE 2.</strong> '''The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).'''<br/>Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank. </em> </p> | ||
| − | <div style="text-align:center">[[Image:T--Tongji_China--202_t.jpg| | + | <div style="text-align:center">[[Image:T--Tongji_China--202_t.jpg|650px]]</div> |
<p style="width:600px; margin-left:100px; margin-bottom:60px; | <p style="width:600px; margin-left:100px; margin-bottom:60px; | ||
text-align:justify "><em><strong>FIGURE 3.</strong> '''From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM. </em> </p> | text-align:justify "><em><strong>FIGURE 3.</strong> '''From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM. </em> </p> | ||
Revision as of 23:58, 27 October 2020
Prm RBS HrtR Ter
Part of heme biosensor system. HrtO repressor, stop repressing when heme exists
Usage and Biology
FIGURE 2. The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank.
FIGURE 3. From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 364
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 364
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 364
- 1000COMPATIBLE WITH RFC[1000]