Difference between revisions of "Part:BBa K3657002"
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Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)
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Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657003, BBa_K3657004) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)
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Revision as of 23:42, 27 October 2020


RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.


This part is the Uracil binding Pumby Domain.


Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657003, BBa_K3657004) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]