Difference between revisions of "Part:BBa K3411030"
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To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate. | To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate. | ||
| − | Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created | + | Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created Part BBa_K3411030 for the PdcuS promoter since the HKUST-Rice 2015 iGEM team was not required to create the basic parts for the corresponding composite part. |
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Revision as of 23:11, 27 October 2020
PdcuS
To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.
Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created Part BBa_K3411030 for the PdcuS promoter since the HKUST-Rice 2015 iGEM team was not required to create the basic parts for the corresponding composite part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]