Difference between revisions of "Part:BBa K3657016:Design"

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===Design Notes===
 
===Design Notes===
 
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The part was designed based on the original Broccoli aptamer with the use of <a href="">DesignSimple</a> in order to exclude the BsmbI cutting site.
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The part was designed based on the original Broccoli aptamer with the use of <a href="https://github.com/igemsoftware2020/Heidelberg_2020/blob/main/CoNcoRDe/CoNcoRDe/rnasupport/scripts/design_simple.py">DesignSimple</a> in order to exclude the BsmbI cutting site.
 
<p>Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly <i>in vitro</i>.</p>
 
<p>Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly <i>in vitro</i>.</p>
 
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Revision as of 23:17, 27 October 2020


Broccoli Aptamer without BsmbI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part was designed based on the original Broccoli aptamer with the use of DesignSimple in order to exclude the BsmbI cutting site.

Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly in vitro.

Source

Synthetic

References