Difference between revisions of "Part:BBa K3505022"

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This part is used in <bbpart>BBa_K3505038</bbpart>
 
This part is used in <bbpart>BBa_K3505038</bbpart>
  
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====Sequence and Features===
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===Sequence and Features===
 
<partinfo>BBa_K3505022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505022 SequenceAndFeatures</partinfo>
 +
===Source===
 +
Georgios Skretas [1]
  
 
===References===
 
===References===
 
*[1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. <em>Journal of Molecular Biology</em>, 429(12), pp.1800-1816.
 
*[1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. <em>Journal of Molecular Biology</em>, 429(12), pp.1800-1816.

Revision as of 00:14, 28 October 2020


RraA- Regulator of ribonuclease activity A GB compatible with B2-B5

Level 0 CDS

Fig.1:RraA


Usage and Biology

The Regulator of ribonuclease activity A (RraA), a repressor of the mRNA-degrading ability of the E. coli RNase E. [1] This protein leads to better expression of non omologous membrane proteins in E. coli.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The CDS has position B2-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.4:Level 0 RraA U2 C2 Digested with EcoRI, PstI Expected bands 2029, 564

Experimental Use and Experience

This part is used in BBa_K3505038

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Georgios Skretas [1]

References

  • [1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. Journal of Molecular Biology, 429(12), pp.1800-1816.