Difference between revisions of "Part:BBa K3505022"

Revision as of 20:49, 27 October 2020

RraA- Regulator of ribonuclease activity A GB compatible with B2-B5

Level 0 CDS

Fig.1:RraA

Usage and Biology

The Regulator of ribonuclease activity A (RraA), a repressor of the mRNA-degrading ability of the E. coli RNase E. [1] This protein leads to better expression of non omologous membrane proteins in E. coli.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The CDS has position B2-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.4:Level 0 RraA U2 C2 Digested with EcoRI, PstI Expected bands 2029, 564

Experimental Use and Experience

This part is used in BBa_K3505038

=Sequence and Features

References

• [1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. Journal of Molecular Biology, 429(12), pp.1800-1816.