Difference between revisions of "Part:BBa K3331018"
 
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We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.
 
We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.
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<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 4:</b> Quantitative result of GFP and RFP fluorescent intensity of the light-controlling switch.(The blue dotted line represents the time point of starting red light treatment and heat at 42℃)]]</div>
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<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b>Experimental medium. 6-1 is blank control ,6-4 is experimental group.
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The protocol of the experiment is list below.]]</div>

Latest revision as of 20:33, 27 October 2020


E.coli BAD +MazF

This is a suicide switch controlled by an arabinose operon. When arabinose is present, the bacteria activate the suicide switch and die.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2001
    Illegal BamHI site found at 1548
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.

Figure 1:Experimental medium. 6-1 is blank control ,6-4 is experimental group. The protocol of the experiment is list below.