Difference between revisions of "Part:BBa K3331005"
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===Characterization===
 
===Characterization===
  
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We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.
+
In the figure, the bacteria in this plate contained plasmids of pBAD-MazF. Three of 6-1
 +
groups were the control group without arabinose induction, and three of 6-4 groups were  
 +
the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the
 +
group with * is 10^4, and the dilution factor of the group without * is 10^6.
 
<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b> Experimental medium. 6-1 is blank control ,6-4 is experimental group.]]</div>
 
<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b> Experimental medium. 6-1 is blank control ,6-4 is experimental group.]]</div>

Revision as of 00:04, 28 October 2020


nodA(MazF)

ndoA (ydcD, mazF) [ Bacillus subtilis subsp. subtilis str. 168 ]


Encodes EndoA, an endoribonuclease that inactivates cellular mRNAs by cleaving them at specific, but frequently occurring sites. EndoA has similar cleavage specificity to MazF and PemK and yields cleavage products with 3′‐phosphate and 5′‐hydroxyl groups, typical of EDTA‐resistant degradative RNases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

In the figure, the bacteria in this plate contained plasmids of pBAD-MazF. Three of 6-1 groups were the control group without arabinose induction, and three of 6-4 groups were the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the group with * is 10^4, and the dilution factor of the group without * is 10^6.

Figure 1: Experimental medium. 6-1 is blank control ,6-4 is experimental group.