Difference between revisions of "Part:BBa K3331005"
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We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression. | We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression. | ||
| − | <div>[[ | + | <div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b> Experimental medium. 6-1 is blank control ,6-4 is experimental group.]]</div> |
Revision as of 20:30, 27 October 2020
nodA(MazF)
ndoA (ydcD, mazF) [ Bacillus subtilis subsp. subtilis str. 168 ]
Encodes EndoA, an endoribonuclease that inactivates cellular mRNAs by cleaving them at specific, but frequently occurring sites. EndoA has similar cleavage specificity to MazF and PemK and yields cleavage products with 3′‐phosphate and 5′‐hydroxyl groups, typical of EDTA‐resistant degradative RNases.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.
