Difference between revisions of "Chassis/B.subtilis Strains"
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==Introduction== | ==Introduction== | ||
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+ | [[Image:Bsub-1.gif|right|frame| ''B. subtilis'' swimming in LB medium]] | ||
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+ | ''Bacillus subtilis'' is a commonly used laboratory bacterium. It isoften considered the gram-positive ''E.coli'' beacuse fo the amount of use it has recieved and the amount of knowledge there is about the organism. | ||
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+ | Its genome has been sequenced (as has the genome of at least one strain - 168) and it has been used at iGEM since 2007. It is gradually coming in to favour, particularly in the UK as a potnetial host for many synthetic systems and devices, often due to the characteristics that make it different to ''e.coli'' | ||
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+ | The lack of parts for ''B. subtilis'' on the registry have reduced the common use of ''B. subtilis'' as a chassis, but with the increased use of ''B. subtilis'', particularly by iGEM teams, it may be soon become a third major host for the registry. | ||
Revision as of 04:01, 30 October 2008
Bacillus subtilis
B.subtilis strains | B. subtilis chassis characterisation | B. subtilis specific parts | Key Parts and Devices |
Introduction
Bacillus subtilis is a commonly used laboratory bacterium. It isoften considered the gram-positive E.coli beacuse fo the amount of use it has recieved and the amount of knowledge there is about the organism.
Its genome has been sequenced (as has the genome of at least one strain - 168) and it has been used at iGEM since 2007. It is gradually coming in to favour, particularly in the UK as a potnetial host for many synthetic systems and devices, often due to the characteristics that make it different to e.coli
The lack of parts for B. subtilis on the registry have reduced the common use of B. subtilis as a chassis, but with the increased use of B. subtilis, particularly by iGEM teams, it may be soon become a third major host for the registry.
Benefits vs Challenges
Commonly used strains
168
Bacillus subtilis strain 168 is one of the most commonly used in the laboratory and its specific genome has been sequenced.
168 tends to be the traditional gram-positive lab worhhorse.
It was used by the Imperial 2008 iGEM team in the project Biofabricator subtilis and proved to be a useful and reliable chassis, being easy to transform, highly motile and generally easy to work with.
This chassis currently provides the basis for the B. subtilis chassis characterisation data
Py79
Like 168, Py79 is also a lab strain of bacillus subtilis.
It is commonly used in research and also is a general workhorse strain.