Difference between revisions of "Part:BBa K3365009:Design"

Latest revision as of 19:23, 27 October 2020

Lure3 sequence downstream of pBAD

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 74
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 56

Design Notes

The lure3 sequence is contained in the reverse primer.

Source

The lure3 sequence is predicted by our off-target predictor software built in 2019 and ligated to the promoter of the L-arabinose operon of E. coli (pBAD) by PCR.

References

[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30.

Revision as of 19:23, 27 October 2020 (view source) Little ghost (Talk \| contribs) (→‎Source) ← Older edit		Latest revision as of 19:23, 27 October 2020 (view source) Little ghost (Talk \| contribs) (→‎References)
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	===References===		===References===
		+	[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30.